This study was carried out to evaluate the antibacterial activity of the fruit and leaf extracts of bitter melon (Momordica charantia, MC) on ATCC bacteria. The methanol and petroleum ether crude extracts of the MC fruits and leaves were evaluated for antibacterial activity on five ATCC microorganisms (Pseudomonas aeruginosa, Escherichia coli, Candida albicans, Streptococcus D (Streptococcus group D), and Staphylococcus aureus) using the disc diffusion method. The results showed that the crude extracts exhibited antibacterial activity against all tested microorganisms, and the methanol extract presented a relatively broader spectrum of antibacterial activity than the petroleum ether extracts. Methanol extract of the MC fruit gave the broadest spectrum of antibacterial activity with inhibition of all microorganisms tested. In particular, there was no synergistic or complementary effect when mixing extracts (from either methanolic or petroleum ether solvent) of leaves and fruit in equal concentrations. The results suggest that methanolic and ether petroleum extract of M.charantia has antibacterial activity against the tested microorganisms.