The green material has been of great interest to scientists in the field of green chemistry. Among the green materials, biodegradable active film from biomass is considered as one of the most potential materials thanks to its popularity in life. Therefore, active packaging films has been synthesized from chitosan, and gelatin incorporating Hass avocado extracts for beef preservation. The effect of different concentrations of extracts (0, 5, and 10) % added to the film was investigated. The FTIR results showed that the presence of Hass avocado extract did not change the film structure. However, the film containing 10 % extract demonstrated significant effectiveness in controlling microbial density, as compared to samples coated with the films containing 0 % Hass avocado extract (GCF). Beef samples coated with the film containing 10 % Hass avocado extract (HGCF10) and stored at the temperature of (4-5) ℃ can inhibit Coliforms bacteria and improve the sensory quality of the sample for up to 6 days of storage. However, beef stored for longer than 14 days developed a higher density of Coliforms and lost its sensory stability.