The study was conducted to figure out parasitic and bacterial pathogens infected frog (Rana sp.) with hydrocoelom. Results on 110 frog samples collected in Dong Thap province from April 2021 to January 2022 showed that frogs were often infected by 9 Cilliophora species such as Balantidium coli, B. elongatum, B. entozoon, B. honghuensis, Cepedea longa, C. magna, Opalina natalensis, O. ranarum, and Zelleriella binucleata. The bacterial identification results identified 77 bacterial isolates, which were 2 species including Aeromonas hydrophila and Pseudomonas putida. P. putida had the highest infection rate (74%). Histological results showed that liver tissue had signs of cellulitis, damaged structures, pancreatic islet areas were congested necrosis, and loss of structure. The renal tissue showed the expression of swelling, inflammation, and congestion. The renal tubular structures and Bowman's capsules were altered, ruptured, and enlarged. The intestinal tissue showed that epithelium was ruptured, cells were loss of structure, and Cilliophora species were present. The folds of the intestinal mucosa were stuck and many places of congestion. The lung tissue showed that inflammation, hemorrhage, and congestion in the lungenbronchus and septum structures.