Acid pretreatment is also considered a s an optimal solution for the hydrolysis of lignocellulosic biomass by removing lignin and part of hemicellulose in pine wood. However, there are still a number of organic compounds derived from hemicellulose such as xylobiose, short-chain xylooligosaccharides and xylan that have not been removed and hinder the hydrolysis ability of the cellulase enzyme. Studying the interaction between enzymes and surfactants in the hydrolysis of cellulose and hemicellulose from pine wood pretreated with formic acid helps enhance enzyme accessibility and promotes faster saccharification. In this study, the enzymes Cellic Ctec 2 and Cellic Htec 2 together and the surfactant Triton X-100 were used as biocatalysts for hydrolysis cellulose and xylan in pine wood. The surface response model (R2 = 0.99 and R2 – adj = 0.97) shows that the cellulase Cellic Ctec2 (p < 0.0001) has a relatively large influence on the ability to hydrolyze cellulose into glucose. The results show that surfactants are involved in the hydrolysis of celluloseand hemicellulose in pine wood after acid pretreatment, helping the enzyme operate stably for a long time, increasing the ability of glucose recovery at the highest value of 295.2g/L.
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