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  • Công bố khoa học và công nghệ Việt Nam

62.39

Các công nghệ xử lý sinh học, xúc tác sinh học; lên men

Huỳnh Hoàng Như Khánh, Bùi Minh Lý, Lê Quang Huấn, Mikhail Kusaykin, Yu V Dubrovskaya, Cao Thị Thúy Hằng(1), Phạm Đức Thịnh, Ngô Thị Duy Ngọc

Sàng lọc enzyme O-Glycoside Hydrolase ở động vật không xương sống từ biển Việt Nam

Screening of O-glycoside hydrolase from Vietnam marine invertebrates

Công nghệ Sinh học

2013

2

275-283

1811-4989

The distribution of O-glycoside hydrolase (fucoidanase, polymanuronic lyase, laminaranase) in 84 species of marine invertebrates in Vietnam was studied. After collected, species names of the invertebrates were identified and samples were kept at 4°C during transfering to the laboratory. The digestive tracts, hepatopancreas, crystalline stalks and eggs were separated, homogenenised in a buffer and then, concentrated to obtain a crude extract. The crude extracts were applied on a Sephadex G-25 column (1.2 x 20 cm), then enzymological activity was screened with substrates from brown algae, including fucoidan, polymanuronic acid and laminaran. It was shown that O-glycoside hydrolase was widespread in the animals analyzed. Some mollusks, annelid and echinoderm species such as Lambis sp. (gastropoda), Septifer biocularis, Maleus maleus.. .(bivalvia) could probably serve as sources of enzymes that degrade polysaccharides from seaweeds as well as O-glycoside hydrolase.

TTKHCNQG, CVv 262