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  • Công bố khoa học và công nghệ Việt Nam

68.03.03

Công nghệ sinh học trong nông nghiệp

Trịnh Minh hợp, Nguyễn Thị Nha, Trịnh Thị Vân Anh, Thái Thị Lệ Hằng, Đặng Minh Tâm, Lê Trọng Tình, Trần Thanh Hùng, Phan Hữu Tôn(1), Chu Hoàng Hà, Lê Trần Bình

Kết quả chuyển gien Bt vào cây bông bằng vi tiêm vào bầu nhuỵ thông qua đường ống phấn

Results of Bt gene transformation into cotton plant by microinjecting via polien tube pathway

Nông nghiệp & Phát triển nông thôn

2013

19

43-50

1859-4581

Bông Bt, Kết quả, Di truyền, Phương pháp, Chuyển gien thông qua đường ống phấn

From 2007 to 2012, at Nha Ho Research Institute for Cotton and Agricultural Development, CryIAc gene were transformed into 4 cotton cultivars (C118, LRA5166, MCU9 and TMl) by microinjecting via pollen tube pathway (PTP). The study determinro two essential parameters, including microinjection time was from 6 to 8 hours of the second day after blooming and length of needle to syringe into ovary of C118, LRA5166, MCU9 and TM1 cultivars were 0.72, 0.62, 0.74 and 0.78 em respectively. Three solutions containing 10, 20 and 30 ug/ml DNA plasmid (pIBT-CryIAc) were microinjected into 14,375 flowers and produced 144,395 T0 generation transgenic seeds. Screening and evaluating transgenic plants (by kanamycin resistance, PCR and insect bioassay) 80 bollworm resistant transgienic lines have been obtained at T2 generation (7 lines of LRA5166, 64 lines of MCU9 and 9 lines of TM1), 50 lines of which were high bollworm resistance (7 lines of LRA5166, 38 lines of MCU9 and 5 lines of TM1). The average transgenic plant frequency of this study was 0.1 percent. The transgenic plant frequency of C118, LRA5166, MCU9 and TM1 cultivars were 0, 0.03, 0.54 and 0.05 percent respectively. The transgenic plant frequency of 10, 20 and 30 ug/ml DNA plasmid concentrations were 0, 0.26 and 0.05 percent respectively.

TTTTKHCNQG, CVv201

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