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Hoàng Minh Đức(1)

Sự hiện diện, khả năng kháng kháng sinh và đặc tính sinh học phân tử của vi khuẩn Staphylococcus aureus phân lập từ thịt lợn và thịt gà bày bán tại huyện Gia Lâm, thành phố Hà Nội

Occurrence, antibiotic resistance profile and molecular characterization of Staphylococcus aureus isolated from pork and chicken meat sold in Gia Lam District, Hanoi City

Kiểm nghiệm và An toàn Thực phẩm

2024

2

78-88

2615-9252

Staphylococcus aureus, Độc tố, MRSA, Kháng kháng sinh

Staphylococcus aureus, Toxin, MRSA, Antibiotic resistance.

Staphylococcus aureus là một trong các vi khuẩn quan trọng nhất gây bệnh cho cả người và động vật. Thực phẩm, đặc biệt là thịt được coi là nguồn chính đưa vi khuẩn vào cơ thể con người. Kết quả nghiên cứu cho thấy tỷ lệ nhiễm S. aureus ở các mẫu thịt lợn và thịt gà lần lượt là 24% và 16%. Các chủng S. aureus phân lập đươc trong nghiên cứu này có tỷ lệ kháng cao nhất với penicillin (90%) và ampicillin (75%); thấp nhất với meropenem (5%) và linezoid (10%). Đặc biệt, tất cả các chủng S. aureus phân lập có khả năng kháng từ một loại kháng sinh trở lên và 65% số chủng phân lập được xác định là các chủng đa kháng. Kết quả phát hiện các gen độc lực cho thấy tất cả các chủng phân lập đều mang gen spa và 15% số chủng mang gen mecA. Tỷ lệ chủng phân lập mang gen sea, seb, sed, see lần lượt là 20%, 5%, 15%, 5%.

Staphylococcus aureus (S. aureus) is one of the most important zoonotic pathogens causing diseases for both humans and animals. Food, particularly meat, is considered the main vector for the transmission of this bacterium to humans. The findings of this study indicate that S. aureus contamination rates of pork and chicken meat samples were 24% and 16%, respectively. S. aureus isolates showed the highest resistance rates to penicillin (90%) and ampicillin (75%), and the lowest resistance to meropenem (5%) and linezolid (10%). Notably, 100% of the isolates were resistant to at least one antibiotic, and 65% were classified as multidrug-resistant strains. The results of molecular characterization revealed that all S. aureus isolates were positive for spa and 15% carried mecA. The detection rates of sea, seb, sed, and see genes were 20%, 5%, 15%, and 5%, respectively.

TTKHCNQG, CTv 166

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