In the present study, the authors propagated Codonopsis javanica Blume plant derived from the Lang Biang highland of Lamdong province, which is one of the valuable herbal plants and has been proved to be good for the human health. Axillary buds of in vitro stem nodes were used as material source for all e~periments. After 30 days of culture, for the BA experiment, MS medium supplemented with 0.5 mg/l BA, 30 g/l sucrose, 8 g/I agar, pH 5.8 was the best for shoot growth and regeneration (10.40 shoots/explant, height of 2.20 cm). For the experiment using BA in combination with NAA, MS medium containing 0.5 mg/l BA, 0.1 mg/l NAA, 30 g/l sucrose, 8 g/l agar, pH 5.8 was the best for shoot growth and regeneration (17.70 shoots/explant, height of 2.78 cm). Kinetin (0.1 - I mg/l) in combination with NAA at 0.1 mg/l stimulated root regeneration but inhibited shoot growth. The same culture medium with the addition of I g/l activated charcoal stimulated root formation of explants and shoots growth, but was not suitable for shoot regeneration. MS medium containing 0.1 - I mg/I IBA or NAA or IAA, 30 g/I sucrose, 8 g/I agar, pH 5,8 was found to be the most suitable for in vitro rooting. The substrate of coconut fiber powder and substrate of half coconut fiber powder in combination with half sand proved the most suitable for plantlet growth in the greenhouse after 20 days of transplantation, with a survival rate at 100 percent.
Lọc theo danh mục
Lượt truy cập :
30,040,373
Xem toàn văn