Breast cancer is a very common cancer in women, the diagnosis and the therapy of early stage tumors have the potential to decrease morbidity and mortality rate of patients with breast cancer. In recent years, scientists have demonstrated that the cancer cells derived from tumor infiltrating and moving in peripheral blood from a very early stage known as Circulating Tumor Cell (CTC). The detection sensitive genes from CTCs may prove useful for screening, prognostication, monitoring of response to therapy. The goal of this study: Detection hMAM mRNA, Survivin mRNA in peripheral blood of breast cancer patients, correlation between the expression of hMAM mRNA, Survivin mRNA and some other factors in them. Patients and methods: Blood sample obtained from 24 breast cancer patients, 5 healthy volunteers. Using RT-PCR, gene sequencing techniques to discover Survivin, hMAM gene in breast cancer cell lines, cancer tissues and in CTCs. Results: Detection rates hMAM mRNA and Survivin are 14/24 (58.3 percent), 11/24 (45.8 percent) in blood, no expression of hMAM and Survivin in healthy human blood (0/5). The expression rate of two genes related to tumor size and stage of disease (p 0.05). Conclusion: The findings were special genes from breast cancer cells in peripheral blood.
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