Nghiên cứu quy trình chuyển gen vào cây dưa hấu (Citrullus lanatus Thumb.)

Chỉ số đề mục

62.37

Lĩnh vực nghiên cứu

Công nghệ sinh học công nghiệp

Dạng tài liệu

Tác giả

Nguyễn Thị Thanh Nga, Hồ Mạnh Tường, Phạm Thị Vân, Nguyễn Tường Vân⁽¹⁾, Chu Hoàng Hà, Lê Trần Bình

Nhan đề

Nghiên cứu quy trình chuyển gen vào cây dưa hấu (Citrullus lanatus Thumb.)

Nhan đề tiếng anh

Optimisation of genetic transformation procedure for watermelon (Citrullus lanatus Thumb.)

Nguồn trích

Sinh học

Năm xuất bản

2012

Số

3

Trang

389-396

ISSN

0866-7160

Từ khóa

Agrobacterium, Chuyển gen, Dưa hấu, Gen gus, Kanamycin, Citrullus lanatus Thumb.

Từ khóa tiếng anh

Tóm tắt

Tóm tắt tiếng anh

In this study, the binary vector pCB-gusplus carrying nptII and gus - in trans genes as selectable and reportable markers was used to optimize Agrobacterium-mediated gene transformation protocol of four Vietnam watermelon lines. Agrobacterium tumefaciens CV58 habouring pCB-gusplus in the concentration of OD600 0.6-0.8 was infected into 5-7 day cotyledons for 30 minutes and co-cultured for 3 days. The transformed leave materials was cultured in the selectable medium containing MS salts and vitamins supplemented with 1.5 mg/l BAP, 0.5 mg/l IBA, 200 mg/l kanamycin va 500 mg/l cefotaxime. Regenerated shoots was rooted in MS medium added with 0.2 mg/l IBA, 100 mg/l kanamycin and 250 mg/l cefotaxime There was a significant difference in the transformation frequency among watermelon lines. FI TG939 line showed the transformation frequency of 1.87 percent), following F9 Tieulong - Thanglong of 1.85 percent, F1 Kinhkong and F9 Binhthuan lines showed the transformation frequency of 0 percent. The analysis of the obtained lines using GUS histochemical assay and PCR reaction with specific primers of ntpII gene had confirmed the presence of gus gene into 5 transgenic lines.

Kí hiệu kho

TTKHCNQG, CVv27

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