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Lê Ngọc Hùng, Đỗ Ngọc Thúy, Nguyễn Đức Anh, Phùng Văn Trung, Phung Van Trung(1)

NGHIÊN CỨU PHÂN TÍCH CÁC AXÍT CHLOGENIC TRONG HẠT CÀ PHÊ XANH THU THEO THỜI GIAN CHÍN BẰNG THIẾT BỊ HPLC-QTOF

ANALYSIS OF CHLOROGENIC ACIDS IN THE GREEN COFFEE BEANS OF DIFFERENT COLLECTION TIMES BY HPLC-QTOF TECHNIQUES

Tạp chí Phân tích Hóa, Lý và Sinh học

2024

03

17-24

Chlorogenic acids (CGAs) constitute a large family of esters and are present in many medicinal plants. CGAs play an important role in modern traditional medicine, but only some of this family’s pure compoundshave been thoroughly researched and shown good pharmaceutical effects. The green coffee beans (CFX)contained ~ 11% of CGAs depending on the species, cultivation and harvested conditions. The quatitativeanalysis could show the presence of separate CGA compounds. Instead of using expensive pure CGA compounds as analysis standard, the HRMS-QTOF detector could unequivocally assign the compound identity while the coupled HPLC could effectively separate the CGAs . In this study, the HPLC-QTOF technique was used to qualitatively determine the CGAs present in the green coffee beans. Ít showed that the CFX harvested in different times have the same CGAs profiling.

Chlorogenic acids (CGAs) constitute a large family of esters and are present in many medicinal plants. CGAs play an important role in modern traditional medicine, but only some of this family’s pure compoundshave been thoroughly researched and shown good pharmaceutical effects. The green coffee beans (CFX)contained ~ 11% of CGAs depending on the species, cultivation and harvested conditions. The quatitativeanalysis could show the presence of separate CGA compounds. Instead of using expensive pure CGA compounds as analysis standard, the HRMS-QTOF detector could unequivocally assign the compound identity while the coupled HPLC could effectively separate the CGAs . In this study, the HPLC-QTOF technique was used to qualitatively determine the CGAs present in the green coffee beans. Ít showed that the CFX harvested in different times have the same CGAs profiling.

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