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  • Công bố khoa học và công nghệ Việt Nam

62.39

Các công nghệ xử lý sinh học, xúc tác sinh học; lên men

Nguyễn Thị Lâm Đoàn, Van Hoorde Koenraad, Cnockaert Margo, Lê Thanh Bình, Vandamme Peter, Phùng Thị Hằng(1)

Nghiên cứu quần xã vi khuẩn trong nem chua bằng phương pháp không phụ thuộc nuôi cấy

Studying on bacterial community in fermented foods by using polymerase chain reaction denaturing gradient gel electrophoresis (PCR - DGGE)

Khoa học và Công nghệ (Viện Hàn lâm Khoa học và Công nghệ Việt Nam)

2015

2

157-168

0866-708X

Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting was recently introduced into food microbiology. PCR-DGGE were used to investigate bacterial communities developed in Hanoi and Thanhhoa nem chua. Total microbial DNAs were extracted directly from 10 nem chua samples. PCR of V3 168 rDNAs were subjected to DGGE analysis. The results have showed that 10 nem chua samples have different bands involved to microbial species in nem chua. 53 bands were collected. Then, these bands were cut, purified, sequenced and compared to known sequence database in EMBL banle The results indicated that there are mostly belong to lactic acid bacteria such as genus Lactobacillus, Lactococcus, Pediococcus, Leuconostoc, Vagacoccus, Enterococcus, Weissella, remains only two as Macrococcus and Psychrobacter found in Hanoi and Thanhhoa nem chua. The results also indicated Lactobacillus are mainly member in nem chua and they play especially important role in producing specfic aroma, bacteriocin to improve the quality and preservation of nem chua as well.

TTKHCNQG, CVv 5