Skin aging is a contuinuous process influenced by many factors such as free redicals, sun exposure etc. This process is characterized with changes in many skin components, primarily affected by three main cell types: fibroblast, keratinocyte and melaninocyte. Decreased number of keratinocytes leads to less soften skin; while increase or decrease melanine production will lead to imbalance of skin color leading to either: backhead or vitiligo. This suty evaluates the effects of umbilical cord exctracts on proliferation of human keratinocyte and melaline production of melaninocyte in vitro. 25 preparations made of 4 types of extracts included: extracellular exctract of cord tissue; intracellular extract of cord cells; mixtures of these two extracts; intracellular extract of umbilical cord stem cells, were supplemented to culture media of human skin keratinocyte and melaninocyte. Keratinocyte cell proliferation was evalutaetd by MTT technique; melanine production was indirectly evaluated via tyrosine kinase gene expression by Realtime RT-PCR technique. The results show that supplementation of 7.5 percent of preparation 3-5 containing a combination of both extra- and intra-cellular extracts of umbilical cord led to the highest cell proliferation of keratinocyte. However, at this concentration, the 3-5 preparation did not inhibite the expression of tyrosine kinase gene in melaninocyte. These data suggests that umbilical cord extract would be a potential source of material for skin softening product but not brightening one.
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