Evaluated the association between EBV & HPV co-infection in nasopharyngeal non-keratinizing squamous cell carcinoma. Subjects and methods: We designed a cross-sectional study, that was realized on 95 FFPE samples from nasopharyngeal non-keratinizing squamous cell carcinoma, were collected at Hanoi Medical University Hospital from 1/2021 to 6/2022. Results: the average age of nasopharyngeal carcinoma patients is 52,75 (16-87), and the incidence rate of NPC was greater in men than women. In the association between EBV&HPV, the results showed that the rate of EBV infection was 95.8%, and the rate of HPV infection was 15,8% (mainly HPV16 (86,7%)). However, the rate of EBV/HPV co-infection was 13,7%, the rate of only EBV infection was 82,1%, only HPV was 2,1% and 2,1% of patients were not infected with both viruses. Our results showed EBV and HPV co-presence in nasopharyngeal non-keratinizing squamous cell carcinoma, especially including high-risk HPV types. Results suggested that HPV may be co-factor with high risk factor is EBV will be increased NPC progression.