The aim of this study was to develop a polymerase chain reaction (PCR) procedure for detection of Bacillus anthracis in environment. In this study the authors used PCR and primer pairs PA5 and PA8 to amplify a DNA tragment of 596 bps located in pagA gene encoding protein protective antigen (PA). This antigen is a transmission for the lethal factor toxin and edema factor toxin of B. anthracis bacteria. The soil samples used in this study was created by infecting. B. anthracis spores at a concentration of 10 exponent-1 - 10 exponent-3 CFU/g and soil samples contaiminated with B. anthracis proved by biochemical and physiological classification method. DNA templates for PCR reaction were extracted by 2 methods. The fist DNA extraction direct trom the soil and the second DNA extraction direct by EZ-10 Spin Column Soil DNA Mimi Preps Kit by Bio Basic Inc. Using DNA template extracted directly from the soil had gave high sensitivity (the concentration of B. anthracis about 10 exponent -1 CFU/g) and reduced time course to 12 hours for detection. The PCR method can be applied to detect anthrax in environment.
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