Reproduction and meat production are considered as the most economically important traits in livestock production. In developed countries, the molecular markers have been commonly used in the animal breeding program to find the favorable alleles in genes associated with economically important traits. The recent studies showed that RNF212 and NCAPG genes are involved in fertility and meat production in mammals, respectively. In which, T allele of RNF212 P259S gene was reported to significantly associate with a higher recombination rate in cows. Of note, since the meiotic recombination rate correlates positively with the reproductive success of human females, the RNF212 P259S is expected to be a useful marker for increasing the reproductive performance in cattle. In addition, G allele of NCAPG I442M increased the carcass weight of cattle. Therefore, in this study, we conducted the polymorphisms of RNF212 P259S and NCAPG I442M by using PCR-RFLP in 2 crossbred cattle. The results showed that the RNF212 P259S and NCAPG I442M were polymorphic in Red Sindhi and Brahman crossbred cattle. The presence of T allele of RNF212 P259S and G allele of NCAPG I442M are informative for the future breeding of Vietnamese crossbred cattle.