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  • Công bố khoa học và công nghệ Việt Nam

62.37

Công nghệ sinh học công nghiệp

Lê Văn Thức, Lê Thị Bích Thy, Lê Thị Thùy Linh, Hoàng Hưng Tiến, Đặng Thị Diên, Hán Huỳnh Diện, Dương Tấn Nhựt(1)

ứng dụng kỹ thuật ra hoa In vitro để đánh giá khả năng đột biến và chọn lọc màu sắc hoa ở cây mắt mèo (Torenia Fournieri L.) sau chiếu xạ

Application of in vitro flowering technique for evaluating of mutation capacity and selecting flower colour of Torenia fournieri L. following irradiation

Công nghệ sinh học

2014

2

309-317

1811-4989

Gamma irradiation technique combined with tissue culture and in vitro flowering was applied in this study. The results showed that the frequences of variation in plant regeneration from irradiated leaf samples were: 0.67 percent (with 30 Gy dose) and 0.72 percent (with 40 Gy dose) in MV 1 generation; the frequences of variation in irradiated plantlet samples were: 1.05 percent (with 30 Gy dose) and 1.15 percent (with 40 Gy dose) in MV4 generation, the frequences of mosaic were 0.25 percent and 0.08 percent in MV 3 and MV 4 generation, respectively. A total of 16 mutants were selected based on phenotypic variations going thrmlgh screening processes of tissue culture and in vitro flowering. Three promising mutant lines (G40TP1, G40TP2, G30TL1) presented a high genetic stability through generations cultivated in both in vitro and ex vitro conditions when being compared with the controls. These mutant lines G40TP1, G40TP2, G30TL1 had a high potential to become new cultivars. This paper showed that the application of in vitro flowering technique for mutation breeding of Torellia (Torenia fournieri L.) is a significant complementary and effective model for selecting mutants produced by irradiation.

TTKHCNQG, CVv 262