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Y học lâm sàng

Lê Hữu Song, Đào Thanh Quyên, Ngô Tất Trung, Phan Quốc Hoàn, Nguyễn Kim Phương, Phạm Minh Long, Nghiêm Xuân Hoàn, Nguyễn Xuân Chính

So sánh giá trị của PCR đa mồi với cấy máu trong chẩn đoán mầm bệnh gây nhiễm khuẩn huyết

Comparison the value of multiplex-PCR with blood culture in identifying pathogens caused sepsis

Y học Việt Nam

2013

1

32-36

1859-1868

To compare the sensitivity and specificity of multiplex-PCR with blood culture the authors collected 50 samples from suspected to be sepsis patients. Multiplex-PCR and blood culture were parallelindependent performed at the Dept of Molecular Biology and Dept of Microbiology. The results were analysed by physician. 7/22 sepsis patients were positive with multiplex-PCR, 22/22 positive with PeR universal and 12/22 positive with blood culture. 5/12 pathogens identified by blood culture were not in the target pathogens which were designed for multiplex-PCR. The sensitivity of blood culture was higher than multiplex-PCR (54.5 percent vs 31.8 percent ); However, the specificity of blood culture was lower than multiplex-PCR (67.8 percent vs 85.7 percent ). Thus, multiplex-PCR has sensitivity lower but specificity higher than blood culture.

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