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Nguyễn Thị Mai Phương, Trần Vân Khánh, Nguyễn Trọng Tuệ(1), Trần Thị Oanh, Hoàng Đạo Bảo Trâm, Nguyễn Thị Hà, Tạ Thành Văn

Định lượng vi khuẩn Porphyromonas gingivslis trong viêm nha chu bằng kỹ thuật Real Time PCR

Quantification of porphyromonas gingivalis bacteria by real-time PCR technique

Nghiên cứu y học

2014

3

17-26

0868-202X

This study was to quantify Porphyromanas gingiva/is in chronic and aggressive periodontitis patients in comparison with healthy subjects by qPCR, qPCR has been used to quantify Parphyroman as gingivalis in gingival cervical fluid in both disea~ed sites of periodontal patients and healthy sites of healthy subjects. The comparison were done in both diseased sites of chronic periodontitis patients P. gingiva lis 25.10 +/- 4.68 Cycle threshold (Ct) and healthy subjects (P. gingiva/is 33.93 +/- 3.17 Ct) respectively (p 0.05). The comparison were in both diseased sites of aggressive periodontitis patients (P. gingivalis 22.89 +/- 4.63 Ct) and healthy subjects (P. gingivalis 34.38 +/- 2.96 Ct) respectively (p 0.01). In conclusion, Porphyromonas gingiva lis was very common in both chronic and aggressive periodontitis patients (100 percent) and there was a significant difference of Porphyromonas gingivalis quantity in comparison with healthy subjects (p 0.05).

TTKHCNQG, CVv 251