In this study, from the stem sample of Aquilaria crassna which created natural agarwood collected in Huong Khe district - Ha Tinh province, 05 fungi species (M1.2, M4.4, M9.4, H4.11, and H4.15) were isolated and evaluated their ability in application as a bio stimulant that should product high efficiency frankincense. The result of morphological and molecular identification showed that 5 isolated fungi were Penecillum crustosum, Aspergillus nomius, Trichoderma virens, Fusarium solani and Mucor racemosus, respectively. The result also indicated that all isolates were capable of producing extracellular enzymes such as amylase, cellulase and pectinase. In addition, the artificial introduction of the fungal mixtures in 10-year-old Aquilaria crassna plants showed stimulation of agarwood production of those plants, and the N1 mixture (including 05 strains of fungi) gave the best results with a marked size of 2.2 x 36 (cm). The results of this study have initially applied the technology of agarwood production in a sustainable way in Vietnam.