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Đỗ Bá Quốc Thái, Lê Thanh Nguyên, Trần Văn Hiếu(1)

Cố định kháng thể lên hạt latex và ứng dụng phân tích miễn dịch

Antibody immobilization technique onto latex beads and its immunoassay applications

Tạp chí Khoa học và Công nghệ - ĐH Công nghiệp TP. Hồ Chí Minh

2022

01

116-133

2525-2267

Protein cầu nối, Hấp thụ thụ động, Peptide, Polystyrene, Hạt polystyrene latex

Adapter protein, Protein immobilization, Passive absorption, Polystyrene binding peptide, Polystyrene latex bead.

Gắn định hướng kháng thể/protein lên bề mặt hạt latex là một trong những giai đoạn chính, quyết định hiệu quả của xét nghiệm ngưng kết huyết thanh. Hiện nay, ba phương pháp chính được sử dụng bao gồm hấp thụ thụ động, gắn định hướng qua liên kết cộng hóa trị và liên kết ái lực đã có thể giúp cải thiện đáng kể chất lượng của sản phẩm. Chưa dừng lại ở đó, sự phát triển của các kỹ thuật sinh học phân tử hiện đại, đặc biệt là công nghệ protein tái tổ hợp, đã giúp tạo ra các loại protein ngoại lai có khả năng gắn đặc hiệu với bề mặt hạt latex. Bài tổng quan này sẽ điểm qua các yếu tố ảnh hưởng đến quá trình hoạt hóa hạt latex cũng như ưu, nhược điểm của các phương pháp gắn định hướng kháng thể/protein lên bề mặt hạt latex đang được sử dụng rộng rãi. Qua đó, bài viết cũng giới thiệu các phương pháp gắn định hướng kháng thể mới có thể thay thế như sử dụng kháng thể dung hợp đuôi peptide gắn đặc hiệu polystyrene và sử dụng protein cầu nối.

Orientation of antibody/protein onto the latex surface is one of the most important steps, which determines the effectiveness of agglutination test. Currently, three well-known methods are used, including passive absorption, covalent coupling, and affinity immobilization. Besides, improvements in the field of molecular biology, especially the recombination protein technology, help to create a lot of novel proteins which have ability to affinity bind to the material surface. In this review, parameters effecting the latex activation process were summarized. Advantages and disadvantages of each common method were also indicated. In addition, this review also introduced some new methods that could supplant the old ones, including polystyrene-binding peptide conjugated antibody, and adapter protein.

TTKHCNQG, CVv 449

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