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  • Công bố khoa học và công nghệ Việt Nam

62.37; 34.29

Trần Thị Liễu, Vũ Thị Thu Hiền, Nguyễn Tiến Hiệp, Đinh Thị Phòng, Lê Hồ Minh Trí (1)

Tính đa dạng nguồn gen di truyền và cấu trúc quần thể loài thông lá dẹt (Pinus Krempf II Lecomte) - loài đặc hữu hẹp ở Tây Nguyên, Việt Nam bằng chỉ thị ISSR

Genetic variation and population structure in Pinus krempfii Lecomte - endemic species in Tay Nguyen, Vietnam by ISSR markers

Khoa học và Công nghệ (Viện Hàn lâm Khoa học và Công nghệ Việt Nam)

2015

2

169-179

0866-708X

Highland is one of the areas where is abundant with coniferous species of Vietnam. Most of these species are economic and of scientific value. Many coniferous species are in danger of being threatened with extinction, among them is Pinus krempfii species - endemic in Vietnam. Therefore, surveys of species status and the conducting some basic researchs are the scientific basis for the conservation, rational use and sustainable development. Results of the molecular analysis based on ISSR markers were used to study genetic diversity of Pinus krempfii populations collected in Giang Ly, Cong Troi, Hon Giao and Chu Yang Sin. There were 18/26 primers revealed polymorphic. Among 137 fragments were amplified, of which 98 were polymorphic (accounting for 71.53 percent). The genetic diversity is the highest in Chu Yang Sin populations (h = 0.117; I = 0.239; PPB = 62.04; Ne = 1,.226 and He = 0.151) and lowest in Giang Ly population (h = 0.070; I = 0,086; PPB = 13.87; Ne = 1.111 and He = 0.060). The degree of genetic differences identified among populations is low (total varifltion is 20.28 percent) and higher within populations (79.82 percent). A phylogenetic tree based on UPGMA analysis grouped the 70 genotypes into two main clusters with genetic similarity withip clusters ranged from about 65.7 (Pk45 and Pk8) to 100 percent (Pk12 and Pk13; Pk47 and Pk48). These results suggest that Pinus krempfii populations should be paid attention to the conservation of species.

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