Mangrove soil stores a high amount of organic carbon, however one part of this source emitted towards the atmosphere as CO2. The objective of the present study was to determine the influence of biofilm on CO2 fluxes at the soil-air interface of mangrove forest. Fluxes were measured using dynamic closed incubation dark chamber connected to an infra-red gas analyzer (IRGA – Licor 820) at the two main seasons (dry and wet seasons) to find the influence of environmental parameters on the fluxes. In situ measurements showed that CO2 fluxes were 3,98 ± 3,72 mmol m-2 h-1 in the mangrove, and 1,77 ± 1,36 mmol m-2 h-1 in the bare land. However, after having removed 2 mm depth of soil surface, CO2 fluxes were increased 1.28 times in mangroves, and 1.74 times in the bare land. The study results found that the environmental parameters, like temperature, rainfall, and the biofilm on the soil surface have strong impact on the CO2 emission to the atmosphere.