To evaluate the axonal injury and to predict the motor function recovery in supratentorial acute stroke patients. Method: Cross-sectional descriptive study was performed on 28 patients, who had had a supratentorial acute stroke, and had an MRI at Bach Mai hospital from 9/2021 to 8/2022. Result: Almost patients had no change in the signal of the axons, which did not go through the infarct lesion (28,6%), the signals strongly decreased in patients whose axons completely stay inside the infarct lesion (32,1%). FA, ADC index of the axons on the infarct side is lower than on the opposite side (p<0,05). The patient group, who had the axons did not go through the infarct lesion or had no change in axonal signals, were higher at the rate of better motor function recovery after three months than the other groups (respective rates were 39,3% and 25%); the axons group, which stayed completely inside the infarct lesion or had remarkably decreased signal, was very poor at the rate of recovery (respective rates were 32,1% and 39,3%), p<0,05. FA index of the axons in the infarct side of the poor recovery group was smaller than the good recovery group, p<0.001. The ADC index is not different from these groups. Conclusion: The axonal signal, location versus the infarct lesion, and FA index of the axons in the infarct side are the factors that significantly predict motor recovery after three months in acute stroke patients.