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  • Công bố khoa học và công nghệ Việt Nam

62.33

Công nghệ sinh học công nghiệp

Hà Hồng Hạnh, Đỗ Tiến Phát(1), Chu Hoàng Hà, Lê Thị Thu Hiền

Nghiên cứu khả năng tái sinh In vitro thông qua phôi soma của một số dòng cacao ở Việt Nam

In vitro tissue culture via somatic embryogenesis of some selected cacao clones in Vietnam

Công nghệ Sinh học

2013

1

107-114

1811-4989

The vegetative clonal propagation of elite/superior cacao (Theobroma cacao L.) clones has long been recognized.as a potential mean to increase cacao production and genetic improvement. In this study, the authors report results of somatic embryogenesis and plant regeration from floral tissues of 9 elite cacao clones in Vietnam. Staminodes and petal base explants of studied cacao flower buds were placed on primary callus growth (PCO) medium containing with 1 mg/l 2.4-0 and 0.2 mg/l TDZ. Cali were subcultured onto fresh secondary callus growth (SCO) medium supplemented with 50 ml/l coconut water, 1 mg/l kinetin about 14 days before transfering to somatic embryo development medium (EO) supplemented with 1 g/l glucose and 30 g/l sucrose. Using this procedure, somatic embryos trom staminodes of6 clones including TD1, TD3, TD5, TD7, TD8, and TD9 were recovered. The trequency of embryogenesis ranged trom 4.73 (TD9) to 25.64 percent (TD8). TD8 was the only clone showed the availability of somatic embryogenesis from petal base explants. The regenerated embryos are grown to maturity with normal morphology.

TTKHCNQG, CVv 262