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Nguyễn Thị Thanh Huyền, Trần Thị Thanh Thảo, Bùi Đình Nhi(1), Nguyễn Thị Hiền

NGHIÊN CỨU XÂY DỰNG QUY TRÌNH THU NHẬN BACTERIOCIN TỪ CHỦNG Bacillus safensis NBRC 100820

STUDY ON BACTERIOCIN COLLECTION FROM Bacillus safensis NBRC 100820

Tạp chí Phân tích Hóa, Lý và Sinh học

2023

1

157

In this paper, the process of bacteriocin collection from Bacillus safensis NBRC 100820 was studied. By using this procedure, 20 ml of the purified bacteriocin was recovered from 1.0 l of the initial culture medium. The product has a protein concentration of 17.5±0.2 mg/ml, a pH of about 5.0±0.2 and active unit of 732.2 AU/mg. Physico-chemical and microbiological parameters of the recovered bacteriocin after purification showed that product did not contain pathogenic microorganisms E. coli, Salmonella, S. arueus, coliforms, yeasts and molds, and heavy metals Hg, As, Pb, Cd. The product conform to the standards on food additives according to TCVN 10637:2015.

In this paper, the process of bacteriocin collection from Bacillus safensis NBRC 100820 was studied. By using this procedure, 20 ml of the purified bacteriocin was recovered from 1.0 l of the initial culture medium. The product has a protein concentration of 17.5±0.2 mg/ml, a pH of about 5.0±0.2 and active unit of 732.2 AU/mg. Physico-chemical and microbiological parameters of the recovered bacteriocin after purification showed that product did not contain pathogenic microorganisms E. coli, Salmonella, S. arueus, coliforms, yeasts and molds, and heavy metals Hg, As, Pb, Cd. The product conform to the standards on food additives according to TCVN 10637:2015.