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  • Công bố khoa học và công nghệ Việt Nam

62.33

Công nghệ sinh học công nghiệp

Mai Trường, Trần Thị Ngọc Hà(1), Phan Tường Lộc, Lê Tấn Đức, Trần Trọng Tuấn, Đỗ Đăng Giáp, Bùi Định Thạch, Nguyễn Thị Thanh, Phạm Đức Trí, Nguyễn Đức Minh Hùng, Nguyễn Văn Kết, Trần Công Luận(2), Nguyễn Hữu Hổ

Một số kết quả nghiên cứu và tạo nuôi rễ bất định sâm Ngọc Linh (Panax Vietnamensis Ha et Grushv.)

Some results on induction and culture of adventitious roots of Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.)

Công nghệ sinh học

2014

2

355-364

1811-4989

This paper presents some results on the induction and the culture of the adventitious roots of the Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.) on the agar/phytagel or in the liquid media. The aim of this study is to layout the foundation for large-scale propagation of this differentiated tissue for the secondary metabolites production. For inducing adventitious roots, three kinds of tissue, viz., callus from the leaflet disc, the mature somatic embryo with shoot and the compact callus were used. In the one-phase culture, the adventitious roots were generated from the callus on the MS medium with 2 mg/12,4-0 and 10 percent coconut water; the adventitious roots were also generated from the subcultured callus (two-phase culture) on the MS medium with 3 mg/l NAA, MS medium with 3 mg/l NAA, 2 mg/l 2,4-D plus 5 percent coconut water and the B5 medium with 5 mg/l IBA. Besides, the adventitious roots were also obtained from the mature somatic embryos (after removing the tap root) through culture using the 1/2MS medium with 2 - 3 mg/l NNA. The adventitious roots from the very compact callus (via culture on the B5 medium with 5 mg/l IBA) were cultured subsequently for evaluating the mass production capacity via secondary root proliferation. The formation of root primordia from root segments on the White/B5 media was also carried out.

TTKHCNQG, CVv 262