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Phan Thành Đạt(2), Lâm Vĩ Nhã, Nguyễn Nguyền Trân, Phùng Thị Hằng(1), Nguyễn Trọng Hồng Phúc

Mô hình chuột Mus musculus viêm loét dạ dày bởi ethanol, acetic acid và aspirin

Stomach ulcer Mus musculus mice model by alcohol, acetic acid and aspirin

Khoa học (Đại học Cần Thơ)

2021

2

67-77

1859-2333

Acetic acid, Aspirin, Ethanol, Mô hình loét dạ dày

Acetic acid, Alcohol, Aspirin, Stomach ulcer model

Viêm loét dạ dày là bệnh đường tiêu hóa phổ biến và đã có nhiều nghiên cứu, phát đồ phòng trị bệnh này. Nhiều nghiên cứu được thực hiện cận lâm sàng và lâm sàng nhằm tìm ra các loại thuốc phòng trị nhưng mô hình động vật bị loét dạ dày vẫn chưa được thống nhất. Nghiên cứu này thực hiện khảo sát 3 mô hình loét dạ dày cấp tính trên chuột nhắt trắng Mus musculus bởi ethanol (60o , 70o và 80o ), acetic acid (10%, 15% và 20%) và aspirin (350 mg/kg, 500 mg/kg và 650 mg/kg) với các chỉ tiêu đánh giá về tổng độ acid, chỉ số loét theo độ sâu, chiều dài và diện tích vết loét. Kết quả cho thấy cả 3 tác nhân gây loét đều có thể gây loét cấp dạ dày, trong đó, ethanol 70o , acetic acid 10% và aspirin 500 mg/kg là các tác nhân phù hợp để xây dựng mô hình gây loét dạ dày cấp tính.

Stomach ulcers are a common gastrointestinal disease and there have been many studies and strategies to prevent this disease. Many studies have been done subclinical and clinical to find preventive drugs, but the animal model of stomach ulcers has not been agreed. This study was performed to evaluate 3 models of acute gastric ulcer in Mus musculus by ethanol (60°, 70° and 80°), acetic acid (10%, 15% and 20%) and aspirin (350 mg/kg, 500 mg/kg and 650 mg/kg) with the evaluation criteria of total acidity, ulcer index by depth, length and area of the ulcer. The results showed that all 3 ulcer agents could cause stomach ulcers, in which 70° ethanol, 10% acetic acid and 500 mg/kg Aspirin were suitable agents to make stomach ulcer mice models.

TTKHCNQG, CVv 403

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  • [1] Zittel, T.T., Jehle, E.C., & Becker, H.D. (2000), Surgical management of peptic ulcer disease today - indication, technique and outcome,Langenbeck’s Arch. Surg., 385, 84–96. https://doi.org/10.1007/s004230050250
  • [2] Zelickson, M.S., Bronder, C.M., Johnson, B.L., Camunas, J.A., Smith, D.E., Rawlinson, D., Von, S., Stone, H.H., & Taylor, S.M. (2011), Helicobacter Pylori is not the predominant etiology for peptic ulcers requiring operation,Am. Surg., 77(8), 1054–1060. https://doi.org/10.1177/000313481107700827
  • [3] Yeomans, N.D., Lanas, A.I., Talley, N.J., Thomson, A.B.R., Daneshjoo, R., Eriksson, B., AppelmanEszxzuk, S., Langstrom, G., Naesdal, J., Serrano, P., Singh, M., Skelly, M.M., & Hawkey, C.J. (2005), Prevalence and incidence of gastroduodenal ulcers during treatment with vascular protective doses of aspirin,Aliment. Pharmacol. Ther., 22, 795–801. https://doi.org/10.1111/j.1365- 2036.2005.02649.x
  • [4] Wariss, H.M., Mukhtar, M., Anjum, S., Bhatti, G.R., Pirzada, S.A., & Alam, K. (2013), Floristic composition of the plants of the cholistan desert, Pakistan,Am. J. Plant Sci., 4(12), 58–65. https://doi.org/10.4236/ajps.2013.412A1009
  • [5] Søreide, K., Thorsen, K., Harrison, E.M., Bingener, J., Møller, M.H., Ohene-Yeboah, M., & Søreide, J.A. (2015), Perforated peptic ulcer,Lancet., 386, 1288–1298. https://doi.org/10.1016/S0140- 6736(15)00276-7
  • [6] Somchit, M.N., Siti Rahmah, S., Zuraini, A., Ahmad Bustamam, A., Zakaria, Z.A., Somchit, N., & Shamsuddin, L. (2007), Gastroprotective activity of Spirulina platensis in acetic acid and ethanol induced ulcers in rats,J. Nat. Remedies, 7, 37– 42. https://doi.org/10.18311/jnr/2007/192
  • [7] Singer, M. V., Leffmann, C., Eysselein, V.E., Calden, H., & Goebell, H. (1987), Action of ethanol and some alcoholic beverages on gastric acid secretion and release of gastrin in humans,Gastroenterology, 93(6), 1247–1254. https://doi.org/10.1016/0016-5085(87)90252-6
  • [8] Sattar, A., Abdo, A., Mushtaq, M.N., Anjum, I., & Anjum, A. (2019), Evaluation of gastroprotective activity of Myristica fragrans on ethanol-induced ulcer in Albino rats,An. Acad. Bras. Cienc., 91(2), 1–8. https://doi.org/10.1590/0001-3765201920181044
  • [9] Ren, J.S., Kamangar, F., Forman, D., & Islami, F. (2012), Pickled food and risk of gastric cancer - A systematic review and meta-analysis of English and Chinese literature,Cancer Epidemiol. Biomarkers Prev., 21(6), 905–915. https://doi.org/10.1158/1055-9965.EPI-12-0202
  • [10] Reddy, V.P., Sudheshna, G., S, K, A., Saran, S.S., Kumar, S.N., Ram, C.R., & Reddy, K.R. (2012), Evaluation of anti-ulcer activity of Citrullus colocynthis fruit against pylorus ligation induced ulcers in male wistar rats,Int. J. Pharm. Pharm. Sci., 4, 446–451
  • [11] Rahim, N.A., Hassandarvish, P., Golbabapour, S., Ismail, S., Tayyab, S., & Abdulla, M.A. (2014), Gastroprotective effect of ethanolic extract of curcuma xanthorrhiza leaf against ethanolinduced gastric mucosal lesions in SpragueDawley rats,Biomed Res. Int. 2014, 1–12. https://doi.org/10.1155/2014/416409
  • [12] Raeesi, M., Eskandari-Roozbahani, N., & Shomali, T. (2019), Gastro-protective effect of Biebersteinia multifida root hydro-methanolic extract in rats with ethanol-induced peptic ulcer,Avicenna J. phytomedicine, 9(5), 410–418. https://doi.org/10.22038/ajp.2019.12902
  • [13] Pöschl, G., & Seitz, H.K. (2004), Alcohol and cancer,Alcohol & Alcoholism., 39(3), 155–165. https://doi.org/10.1093/alcalc/agh057
  • [14] Ozbakiş Dengiz, G., & Gürsan, N. (2005), Effects of Momordica c-harantia L. (Cucurbitaceae) on indomethacin-induced ulcer model in rats,Turk. J. Gastroenterol., 16, 85–8
  • [15] Oluwabunmi, I., & Abiola, T. (2015), Gastroprotective effect of methanolic extract of Gomphrena celosioides on indomethacin induced gastric ulcer in Wistar albino rats,Int. J. Appl. Basic Med. Res., 5(1), 41–45. https://doi.org/10.4103/2229-516X.149238
  • [16] Niv, Y., Battler, A., Abuksis, G., Gal, E., Sapoznikov, B., & Vilkin, A. (2005), Endoscopy in asymptomatic minidose aspirin consumers,Dig. Dis. Sci., 50(1), 78–80. https://doi.org/10.1007/s10620-005-1281-1
  • [17] Nishino, M., Sugimoto, M., Kodaira, C., Yamade, M., Shirai, N., Ikuma, M., Tanaka, T., Sugimura, H., Hishida, A., & Furuta, T. (2010), Relationship between low-dose aspirin-induced gastric mucosal injury and intragastric pH in healthy volunteers,Dig. Dis. Sci., 55, 1627– 1636. https://doi.org/10.1007/s10620-009-0920-3
  • [18] Nihar Ranjan, K., Shreechandan, P., Priyadarshini, P., & Sabhya Sampad, S. (2017), Herbal drugs in treatment of peptic ulcer,J.Bio., 6(3), 499–508
  • [19] Madi, N., & Abd al-Barr, A. (2014), Comparative evaluation of the gastroprotective effect of LCarnitine and Omeprazole on ethanol-induced gastric ulcer in male Albino Wister rats,Bull. Egypt. Soc. Physiol. Sci., 34(2), 293–309. https://doi.org/10.21608/besps.2014.34799
  • [20] Kim, Y.-S., Park, H.J., Kim, H., Song, J., & Lee, D. (2019), Gastroprotective effects of Paeonia extract mixture HT074 against experimental gastric ulcers in rats,Evidence-Based Complement. Al-tern. Med. 2019, 1–13. https://doi.org/10.1155/2019/3546258
  • [21] Kim, S.-J., Kim, J.M., Shim, S.H., & Chang, H.I. (2014), Anthocyanins accelerate the healing of naproxen-induced gastric ulcer in rats by activating antioxidant enzymes via modulation of Nrf2.,J. Funct. Foods., 7, 569–579. https://doi.org/10.1016/j.jff.2013.12.028
  • [22] Kamarolzaman, M., Yahya, F., Mamet, S., Jakius, K., Mahmood, N., Shahril, M., Mohtarrudin, N., Suhaili, Z., & Zakaria, Z. (2014), Gastroprotective activity and mechanisms of action of Bauhinia purpurea Linn (Leguminoseae) leaf methanol extract,Trop. J. Pharm. Res., 13(11), 1889. https://doi.org/10.4314/tjpr.v13i11.17
  • [23] Jabeen, Q. (2018), Chemically-induced peptic ulcer: Gastroprotective effects of peach fruit.,Curr. Trends Gastroenterol. Hepatol., 1, 22–30. https://doi.org/10.32474/CTGH.2018.01.000107
  • [24] Bekemeier, H. (1955), Salicylamide and salicylic acid poisoning in cats, in comparison with other animals,[WWW Document]. https://chem.nlm.nih.gov/chemidplus/rn/50-78-2
  • [25] Gul, H., Abbas, K., & Qadir, M.I. (2015), Gastroprotective effect of ethanolic extract of Mentha longifolia in alcohol- and aspirin-induced gastric ulcer models,Bangladesh J. Pharmacol., 10, 241–245. https://doi.org/10.3329/bjp.v10i1.21689
  • [26] Goldstein, J.L., Lowry, S.C., Lanza, F.L., Schwartz, H.I., & Dodge, W.E. (2006), The impact of lowdose aspirin on endoscopic gastric and duodenal ulcer rates in users of a non-se-lective nonsteroidal anti-inflammatory drug or a cyclooxygenase-2-se-lective inhibitor,Aliment. Pharmacol. Ther., 23, 1489–1498. https://doi.org/10.1111/j.1365- 2036.2006.02912.x
  • [27] Goddard, A.F., & Spiller, R.C. (1996), The effect of omeprazole on gastric juice viscosity, pH and bacterial counts,Aliment. Pharmacol. Ther., 10, 105–109. https://doi.org/10.1111/j.1365- 2036.1996.tb00183.x
  • [28] Gabriel, S.E. (1991), Risk for serious gastrointestinal complications related to use of nonsteroidal antiinflammatory drugs,Ann. Intern. Med., 115, 787–796. 10.7326/0003-4819-115-10-787
  • [29] Dashputre, N.L., & Naikwade, N.S. (2011), Evaluation of anti-ulcer activity of methanolic extract of Abutilon indicum Linn leaves in experimental rats,Int. J. Pharm. Sci. Drug Res., 3(2), 97–100
  • [30] C-hari, S., Teyssen, S., & Singer, M. V. (1993), Alcohol and gastric acid secretion in humans,Gut., 34, 843–847. https://doi.org/10.1136/gut.34.6.843
  • [31] Chan, F.K.L., & Leung, W.K. (2002), Peptic-ulcer disease,Lancet., 360, 933–941
  • [32] (2016), Hướng dẫn quy trình kỹ thuật chuyên ngành giải phẫu bệnh, tế bào học,
  • [33] Al, I.A., Alhaider, A.A., Mossa, J.S., Al-sohaibani, M.O., Al-yahya, M.A., Rafatullah, S., & Shaik, S.A. (2008), Gastroprotective effect of an aqueous suspension of Black Cumin Nigella sativa on necrotizing agents-Induced gastric injury in experimental animals,Saudi J. Gastroenterol., 14(3), 128–134. 10.4103/1319- 3767.41731
  • [34] Al-bayati, N.J., Ewadh, M.J., Ijam, A.H. (2015), Gastroprotective and antioxidant potential of Montelukast against acetyl salicylic acid induced gastric ulcer model in male rabbits,Int. J. Curr. Microbiol. Appl. Sci., 4(5), 80–89
  • [35] Akre, K., Ekström, A.M., Signorello, L.B., Hansson, L.E., & Nyrén, O. (2001), Aspirin and risk for gastric cancer: A population-based case-control study in Sweden,Br. J. Cancer., 84(7), 965–968. https://doi.org/10.1054/bjoc.2001.1702
  • [36] Ahmed, N., Ali Khan, M.S., Mat Jais, A.M., Mohtarrudin, N., Ranjbar, M., Amjad, M.S., Nagaraju, B., Faraz, M., Pathan, F., & Chincholi, A. ( (2013), Anti-ulcer activity of sandalwood (Santalum album L.) stem hydro-alcoholic extract in three gastric-ulceration models of Wistar rats.,Bol. Latinoam. y del Caribe Plantas Med. y Aromat., 12(1), 81–91
  • [37] Adinortey, M.B., Ansah, C., Galyuon, I., & Nyarko, A. (2013), In vivo models used for evaluation of potential antigastroduodenal ulcer agents,Ulcers. 2013, 1–12. https://doi.org/10.1155/2013/796405
  • [38] Adefisayo, M.A., Akomolafe, R.O., Akinsomisoye, S.O., Alabi, Q.K., Ogundipe, O.L., Omole, J.G., & Olamilosoye, K.P (2017), Gastro-protective effect of methanol extract of Vernonia amygdalina (Del.) leaf on aspirin-induced gastric ulcer in Wistar rats,Toxicol Reports. 4 (2017), 625–633. https://doi.org/10.1016/j.toxrep.2017.11.004
  • [39] Abebaw, M., Mishra, B., & Asmelashe, D. (2017), Evaluation of anti-ulcer activity of the leaf extract of Osyris quadripartita Decne. (santalaceae) in rats.,J. Exp. Pharmacol. 9, 1–11. 10.2147/JEP.S125383