Pluriamine is a natural amino acid mixture obtained from dried silkworm pupae. In that mixture, there are many important essential amino acids such as leucin, isoleucin, lysine, threonin, cysteine, etc. Pluriamine provides essential amino acids for the body, helping to strengthen the body. The nutritional value of protein is determined by the relationship in quantity and quality of different amino acids in the protein, in which amino acids have growth promoting properties such as: arginine, tryptophan, glutamic acid, proline, cysteine, serine, tysosin. The essential amino acids for the body are: Methionine, lysine, tryptophan, phenylalanine, leucin, isoleucin, threonin, valine. In this study, amino acids from silkworm pupae were extracted using protease enzyme method to obtain a mixture of amino acids and valuable biological peptides, which have potential applications as raw materials in food production. function. The total amino acid content obtained by protease hydrolysis was determined by formol titration method, the result was 22.7 g/l. Analysis of hydrolysis products by TLC chromatography showed that the extract contained high-value amino acids such as lysine, alanine, glutamic...