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  • Công bố khoa học và công nghệ Việt Nam

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Trồng trọt

Khuất Hữu Trung, Kiều Thị Dung(1), Đặng Đình Hoàn

Nghiên cứu đa dạng di truyền tập đoàn lúa kháng đạo ôn bản địa của Việt Nam bằng chỉ thị phân tử SSR (Microsatellite)

Analyzing genetic diversity of native blast disease resistant rice varieties in Vietnam by micro-satellite markers

Hoạt động khoa học

2012

6

84-87

1859-4794

The results using 29 SSR markers for analyzing genetic diversity of 34 native blast disease resistant rice varieties have obtained a total number of 956 DNA bands belong to 162 differences alleles (with an average of 5.58 alleles per SSR loci). PIC value changed from 0.34 to 0.85 with a mean of 0.71. These blast disease resistant rice varieties have relatively high genetic purity, the heterozygosity changed from 0 to 13.79 percent (with a mean of 1.57 percent). Genetic similarity coefficients of 34 varieties were ranging from 0.00 to 0.69. At genetic similarity level of 19 percent showed that 34 varieties were divided into six major groups: Group I and VI only consist of 1 variety. Group II consists of 6 varieties with genetic similarity coefficients were ranging from 0.17 to 0.53. Group III consists of 10 varieties with the genetic similarity coefficients were ranging from 0.10 to 0.50. Group IV consists of 6 varieties with genetic similarity coefficients were ranging from 0.15 to 0.60. Group V consists of 10 varieties with genetic similarity coefficients were ranging from 0.13 to 0.69. Using RM172, RM229, RM257, RM270, RM286 and RM341 markers can accurately identifying for Tan lanh, Khau giang cam, Ole te 10, Khau tan nhe, Khau hut danh and D'le la rice varieties. The results also showed that using SSR markers not only for analyzing genetic diversity, determination genetic relationship, among native blast disease resistant rice varieties but also for subspecies classification at an accurate.

TTKHCNQG, CVv8