Lọc theo danh mục
  • Năm xuất bản
    Xem thêm
  • Lĩnh vực
liên kết website
Lượt truy cập
 Lượt truy cập :  22,408,250
  • Công bố khoa học và công nghệ Việt Nam

Khoa học nông nghiệp

BB

Lê Thị Diễm, Trương Hoài Phong, Hoàng Thanh Tùng, Hoàng Đắc Khải, Vũ Quốc Luận, Đỗ Mạnh Cường, Nguyễn Thị Như Mai, Dương Tấn Nhựt*, Trịnh Thị Hương, Bùi Văn Thế Vinh, Trần Quế, Hoang Thanh Tung(1)

So sánh hiệu quả phát sinh phôi vô tính từ các nguồn mẫu in vitro của cây sâm Ngọc Linh (Panax vietnamensis Ha et Grushv.)

Comparison of somatic embryogenesis efficiency from in vitro explant sources of Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.)

Tạp chí Khoa học và Công nghệ Việt Nam - B

2023

9B

51

Nghiên cứu khảo sát khả năng phát sinh phôi vô tính từ nhiều nguồn vật liệu khác nhau của cây sâm Ngọc Linh (Panax vietnamensis Ha et Grushv.) được thực hiện nhằm chọn được vật liệu thích hợp cho sự phát sinh phôi trong nuôi cấy in vitro. Trong nghiên cứu này, các mẫu in vitro từ mảnh lá, cuống lá, rễ và các phôi rời ở dạng hình cầu, hình tim và có lá mầm được khảo sát trên môi trường MS bổ sung riêng lẻ hoặc kết hợp 2,4-D (2,4-dichlorophenoxyacetic acid: 0,2, 0,5 và 0,7 mg/l) và TDZ (Thidiazuron:0,1, 0,3 và 0,5 mg/l)...

This study on the efficiency of embryogenesis from various explant sources of Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.) was conducted to select the appropriate materials and optimise embryogenesis in vitro. In this study, in vitro explants from leaf fragments, petioles, roots, and embryos (globular, heart-shaped, and cotyledon) were cultured on MS medium supplemented individually or in combination with 2,4-D (2,4-dichlorophenoxyacetic acid: 0.2, 0.5, and 0.7 mg/l) and TDZ (Thidiazuron: 0.1, 0.3, and 0.5 mg/l)...

  • [1] S. Nunes, Li. Marum, N. Farinha, et al. (2018), Embryogenesis & ploidy of hybrid Pinus elliottii × P. caribaea,Plant Cell, Tissue Organ Culture
  • [2] X. Yang, X. Yang, T. Guo, et al. (2018), Embryogenesis f-rom seedlings of Koelreuteria paniculata,Forests
  • [3] C. Ahn, A.R. Tull, P.M. Montello, et al. (2017), Clonal propagation of Chamaecyparis thyoides without PGR,Plant Cell, Tissue Organ Culture
  • [4] G.S. Pullman, K. Bucalo (2014), Pine somatic embryogenesis: Seed & medium protocol analysis,New Forests
  • [5] K. Haliloglu, M. Aydin (2016), Regeneration system f-rom wheat leaf base segments,SpringerPlus
  • [6] S. Bhaskaran, R.H. Smith (1990), Review on regeneration in cereal tissue culture,Crop Science
  • [7] M.G. Prakash, K. Gurumurthi (2010), Somatic embryogenesis in Eucalyptus camaldulensis – explant, age, medium,Plant Cell, Tissue Organ Culture
  • [8] Y.E. Choi, D.C. Yang, E.S. Yoon, et al. (1999), High-efficiency single embryogenesis f-rom P. ginseng cotyledons,Plant Cell Rep.
  • [9] S. Arya, I.D. Arya, T. Eriksson (1993), Rapid multiplication of P. ginseng adventitious embryos,Plant Cell, Tissue Organ Culture
  • [10] W. Chang, Y. Hsing (1980), Plant regeneration f-rom callus of Panax ginseng,Theor. Appl. Genet.
  • [11] H.H. Chung, J.T. Chen, W.C. Chang (2005), Cytokinin-induced embryogenesis in Dendrobium chiengmai pink,In Vitro Cell. Dev. Biol. Plant
  • [12] R. Gill, P.K. Saxena (1992), Direct embryogenesis of peanut (Arachis hypogaea) via TDZ,Can. J. Bot.
  • [13] K.A. Malik, P.K. Saxena (1992), Regeneration in Phaseolus vulgaris via TDZ & BAP,Planta
  • [14] M. Zhang, A. Wang, M. Qin, et al. (2021), Direct and Indirect embryogenesis induction in Camellia oleifera,Front. Plant Sci.
  • [15] H.J. Gladfelter, J. Johnston, H.D. Wilde, et al. (2021), Somatic embryogenesis & cryopreservation of Stewartia species,Plant Cell, Tissue Organ Culture
  • [16] T.H. Przybył, E. Ratajczak, A. Obarska, et al. (2020), Different auxin roles in Picea species embryogenesis efficiency,Int. J. Mol. Sci.
  • [17] S. Avci, E. Can (2006), Efficient embryogenesis f-rom immature inflorescences of Dallisgrass,Prop. Orn. Plants
  • [18] M. Umehara, H. Kamada (2005), Development of embryo proper and suspensor during embryogenesis,Plant Biotechnol.
  • [19] C.M. Liu, Z.H. Xu, N. Chua (1993), Auxin polar transport essential for symmetry in early embryogenesis,Plant Cell
  • [20] N. Sabooni, A. Shekafandeh (2017), Somatic embryogenesis and regeneration of blackberry via TCL,Plant Cell, Tissue Organ Culture
  • [21] T. Isah (2016), Induction of somatic embryogenesis in woody plants,Acta Physiol. Plant.
  • [22] I. Żur, E. Dubas, M. Krzewska, et al. (2015), Hormonal requirements for microspore embryogenesis in triticale,Plant Cell Rep.
  • [23] D.B. Duncan (1995), Multiple range and multiple F tests,J. Biometrics
  • [24] R.L. Peterson, C.A. Peterson, L.H. Melville (2008), Teaching plant anatomy through creative laboratory exercises,NRC Press
  • [25] V.T. Hien, N.P. Huy, B.V.T. Vinh, et al. (2016), Somatic embryogenesis f-rom leaf transverse TCL-derived callus of P. vietnamensis,Vietnam J. Biotechnol.
  • [26] B.V.T. Vinh, V.T. Thuy, T.T. Hien, et al. (2014), Factors affecting somatic embryogenesis of Ngoc Linh ginseng (P. vietnamensis),Vietnam J. Sci. Technol.
  • [27] V.T. Hien, V.M. Luan, N.P. Huy, et al. (2014), Direct somatic embryogenesis f-rom Panax vietnamensis leaf, petiole & rhizome,Academia J. Biol.
  • [28] D.T. Nhut, B.V.T. Vinh, T.T. Hien, et al. (2012), Effects of spermidine, proline and carbohydrates on somatic embryogenesis of Panax vietnamensis,Afr. J. Biotechnol.
  • [29] D.T. Nhut, N.P. Huy, H.X. Chien, et al. (2012), In vitro culture of Vietnamese ginseng thin cell layer explants,Int. J. Appl. Biol. Pharm.
  • [30] M.T. Martinez, M.C. San Jose, A.M. Vieitez, et al. (2017), Propagation of Quercus ilex trees by somatic embryogenesis,Plant Cell, Tissue Organ Culture
  • [31] H.A. Mendez-Hernandez, M.L. Rodriguez, R.N. Avilez-Montalvo, et al. (2019), Signaling overview of plant somatic embryogenesis,Front. Plant Sci.
  • [32] Y. Yang, N. Wang, S. Zhao (2020), WRKY gene involvement in somatic embryogenesis in Panax ginseng,Protoplasma
  • [33] J. Yan, P. Peng, G. Duan, et al. (2021), Plantlet regeneration f-rom somatic embryos of Picea mongolica,Sci. Rep.
  • [34] A.M. Wojcik, B. Wojcikowska, M.D. Gaj (2020), Auxin-mediated genetic network in somatic embryogenesis,Int. J. Mol. Sci.
  • [35] R.J. Rose, Y. Song (2017), Somatic embryogenesis,Encyclopedia of Applied Plant Sciences (2nd Ed.)
  • [36] M.D. Bogdanovic, K.B. Cukovic, A.R. Subotic, et al. (2021), Secondary somatic embryogenesis in Centaurium erythraea,Plants
  • [37] Y. Guan, S.G. Li, X.F. Fan, et al. (2016), Application of somatic embryogenesis in woody plants,Front. Plant Sci.
  • [38] Y. Bao, G. Liu, X. Shi, et al. (2012), Somatic embryogenesis in Rosa hybrida ‘Samantha’,Plant Cell, Tissue Organ Culture
  • [39] P.D. Smitha, K.R. Binoy, S.N. Ashalatha (2020), Secondary somatic embryogenesis in diploid banana cultivars,Int. J. Fruit Sci.
  • [40] H.F. Sakhanokho, E.M. Babiker, B.J. Smith, et al. (2019), High-frequency somatic embryogenesis of Asclepias spp.,Plant Cell, Tissue Organ Culture
  • [41] K.H. Neumann (2000), Some Studies on Somatic Embryogenesis,Institute Booklet
  • [42] G. Raza, M.B. Singh, P.L. Bhalla (2020), Somatic embryogenesis and plant regeneration f-rom soybean,Plants (Basel)
  • [43] C. Stasolla, E.C. Yeung (2003), Recent advances in conifer somatic embryogenesis,Plant Cell, Tissue Organ Culture
  • [44] N.T. Huong, K. Matsumoto, R. Kasai, et al. (1998), In vitro antioxidant activity of Vietnamese ginseng saponin and its components,Biol. Pharm. Bull.