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  • Công bố khoa học và công nghệ Việt Nam

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Công nghệ sinh học công nghiệp

Trịnh Thị Hương, Trần Hiếu, Hồ Thanh Tâm, Nguyễn Hồng Hoàng, Vũ Thị Hiền, Lê Kim Cương, Nguyễn Bá Nam, Nguyễn Phúc Huy, Vũ Quốc Luận, Bùi Thế Vinh, Trần Đình Phương, Trần Công Luận, Chu Hoàng Hà, Dương Tấn Nhựt(1)

ảnh hưởng của một số Elicitor lên sự sinh trưởng và tích lỹ saponin trone rễ thứ cấp sâm Ngọc Linh (Panax Vietnamensis Ha et Grushv) nuôi cấy In vitro

The efects of some elicitors on growth and ginsenoside production of secondary roots of Panax vietnamensis Ha et Grushv. cultured in-vitro

Công nghệ Sinh học

2014

3

477-487

1811-4989

Elicitors are known as compounds that when introduced into a living organism signal the activation or synthesis of another compound. In vitro plant materials are good sources for the production of secondary metabolite and elicitation can be used as one of the effective tools in order to improve the synthesis of these compounds. In this study, abscisic acid, jasmonic acid and salicylic acid were added to the culture medium in order to examine effects of these elicitors on growth and ginsenoside production of secondary roots of Panax vietnamensis. After 4 weeks, results indicated that elicitors strongly inhibited secondary roots growth. However, these elicitors made a contribution to ginsenoside production improvement. Thin layer chromatography (TLC) and High-performance liquid chromatography (HPLC) analyses of the roots cultured on SH medium supplemented with 5 mg/l IBA, 30 g/l sucrose, 8 g/l agar and one of these elicitors showed the presence of ginsenoside MR2, RbI and RgI at different contents. The highest contents of MR2 (2.826445 percent) and total saponin (3.42449 percent) in secondary roots were recorded when explants were cultured on medium containing 200 ul/l jasmonic acid.

TTKHCNQG, CVv 262