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  • Công bố khoa học và công nghệ Việt Nam

76.03.33

Công nghệ sinh học trong y học

Nguyễn Quang Tùng(1), Đỗ Trung Phấn

Nghiên cứu khả năng tạo cụm tế bào của các mẫu tế bào gốc tạo máu

Multilineage differentiation of the cell samples collected from human bone marrow, cord blood and peripheral blood

Tạp chí Y học Việt Nam

2012

SDB

95-100

1859-1868

Quantitative of hematopoietic progenitors is commonly done with the use of colony assays. Purpose: Assessment of number and types of cell colonies were formed from bone marrow, cord blood and peripheral blood stem cell samples. Materials and Method: 49 samples were placed in methylcellulose-based colony assay media. Results: Bone marrow samples were formed 322 + or - 25 colonies/10 exponent 5 nucleated cells, include CFU-GEMM (23.4 percent) and CFU-GM (21.6 percent). CFU-G colonies were highest counted form PBSC samples (33.6 percent) and cord blood were highest archived of BFU-E (43.8 percent). Conclusions: Number of cell colonies and its types were different from sources of cell cultured. There results would be useful for choice of cell sources for transplantion.

TTKHCNQG, CVv46