The purpose of this research was to compare the efficiencies of normal and cut-style pollination methods (CSM) for the production of Fl and BC1 interspecific hybrids of lily. This research included twelve combinations of crosses with six groups (Asiatic-hybrids (N, LA-hybrids (LA), Oriental-hybrids (0), OT-hybrids (OT), L. fonnolongi (F) and L longiflorum Thunb. (L). Two culture methods-ovary slice culture (OSC) and embryo-sac rescue method (ESR) were used to culture the fertilized ovules or embryo-sac after cut-style and normal pollination on plants respectively. The results have indicated that CSM is superior for the generating Fl hybrids. The rate of viable seedpods is high (30.0-65.0 percent). Normal pollination method is superior for generating backcrossing populations with the proportion of viable seedpods peaks at 20.0-87.5 percent. For the interspecific crosses, applying OSC method after 8-10 days after pollination brings the highest rate of germinated ovules (40.7-50.6 percent). When using the ESR method, ovaries should be harvested at the best time from 45 to 60 days after pollination (depending on the crossing type and combination), and embryo could be germinated from various genotype combinations (8.1-21.3 percent success).
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