This study aimed to preliminarily analyze the phytochemical composition and evaluate factors affecting the bioactivity of Centella asiatica (L.) Urb. extracts. C. asiatica sample was collected in a local garden in Tien Giang province under traditional local cultivation conditions; the VietGAP C. asiatica sample was also collected for comparison. The results showed a strong effect of solvents on the phytochemical content and bioactivity of the C. asiatica extract when using the ultrasonic-assisted extraction technique. The ethanol/water (3:2 v/v) solvent provided the highest antioxidant extract, while the ethanol solvent supported the extraction of antibacterial compounds. The appropriate conditions for antioxidant activityguided extraction were determined including ethanol/water (3:2 v/v) solvent, extraction time of 45 min, and temperature of 40 oC. Under these conditions, the total saponin content, total phenolic content, and total flavonoid content of the extract were 24.15 ± 0.04 mg oleanolic/g, 22.17 ± 0.63 mg GAE/g, and 21.25 ± 0.46 mg RE/g, respectively. The C. asiatica sample under VietGAP had a higher amount of total saponins than the C. asiatica sample collected in the local garden, while the latter contained higher amounts of phenolics and flavonoids