Lọc theo danh mục
  • Năm xuất bản
    Xem thêm
  • Lĩnh vực
liên kết website
Lượt truy cập
 Lượt truy cập :  23,214,706
  • Công bố khoa học và công nghệ Việt Nam

68.41; 34.25.15

Lê Lập, Lê Văn Sơn, Võ Thành Thìn, Lê Đình Hải(1), Nguyễn Viết Không

Vị trí phân loại của các chủng Clostridium Perfringens phân lập tại Việt Nam dựa vào trình tự gien 16S rRNA

Phylogenetic positions of Clostridium perfringens strains isolated from Vietnam based on 165 rRNA gen sequences

Khoa học Kỹ thuật Thú y

2012

1

68-74

0868-2933

The nucleotide sequence of 645bp fragments on 16S rRNA of 5 C. perfringens strains isolated from diarrhea-enteritis goats and sheep reared in Vietnam were completely identical; they were highly similarity (99,8 - 100 percent); in which the 16S rRNA gen sequences of three strains: CD4 (type D) isolated from the North, DKH (type D) from Khanh Hoa va CV58 (type A) from Ninh Thuan were totally homologue (100 percent), demonstrated that these gens were highly conserve and homologue among C. perfringens strains of different toxinotypes and different isolated places; among the other strains (CV58, CV135 and type C), they differed by 1 nucleotide, or level of similarity 99.8 percent. This difference could be a random point mutation, these results suggest that the 645bp fragments on 16S rRNA of 5 C. perfringens strains isolated from Vietnam are homologue. Phylogenetic tree analysis based on the comparison of 16S rRINA gen sequences of genus Clostridium (Collins et al., 1994; Sasaki et aI., 2001, 2002), the strains isolated from Vietnam belong to the position of genus Clostridium, Cluster l. Clade l-a, species C. perfringens.

TTKHCNQG, CVv65