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Cao Thị Thúy Hằng(1), Trần Thị Thanh Vân, Nguyễn Thị Thuận, Trần Nguyễn Hà Vy, Võ Mai Như Hiếu, Phạm Đức Thịnh*, Nguyễn Thị Khánh Vy

Khảo sát điều kiện hoạt động của Viscozyme trên rong nâu Sargassum mcclurei để thu nhận fucoidan

Survey of the Viscozyme’s hydrolysis conditions on brown seaweed Sargassum mcclurei for fucoidan assisted-extraction

Tạp chí Khoa học và Công nghệ Việt Nam - B

2022

8B

22

rong nghiên cứu này, điều kiện hoạt động của Viscozyme được khảo sát nhằm thu nhận fucoidan với hàm lượng cao từ rong nâu Sargassum mcclurei thu tại vùng biển Khánh Hòa. Các điều kiện khảo sát gồm nồng độ Viscozyme, tỷ lệ dịch chiết/rong nguyên liệu và thời gian chiết. Kết quả cho thấy, ở điều kiện nồng độ enzyme 1% (v/w), tỷ lệ dịch chiết/rong nguyên liệu là 25/1 (v/w), thời gian chiết 24 giờ thì hàm lượng fucoidan thu nhận được đạt 4,27%. Kết quả phân tích fucoidan chiết từ loài rong nâu S. mcclurei cho thấy fucoidan thu nhận được thuộc nhóm galactofucan.

In this study, the hydrolysis conditions of Viscozyme on brown seaweed Sargassum mcclurei collected in the Khanh Hoa sea were investigated to obtain fucoidan with a high yield. The survey conditions include the concentration of Viscozyme, the extract solution:raw seaweed ratio, and extraction time. The results showed that in the condition of 1% (v/w) enzyme concentration, the ratio of enzyme/raw seaweed was 25/1 (v/w), extraction time was 24 hours, and the obtained fucoidancontent reached 4.27%. The fucoidan analysed results showed that the obtained fucoidan belongs to the galactofucan group.

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