With economic, technical and environmental benefits, the use of Reclaimed Asphalt Pavement (RAP) has become more and more popular in recent years. In order to restore the rheological and other properties of aged bitumen recovered from RAP (RAP bitumen), rejuvenator is commonly used during the asphalt mixtures production containing high RAP content. This paper presents a laboratory study on the effect of petroleum-based rejuvenator (HS1 rejuvenator) on the rheological properties of bitumen blends. The bitumens evaluated include virgin bitumen (60/70 penetration grade), RAP bitumen and blends bitumen (including virgin bitumen, RAP bitumen with a recycled binder ratio of 0.3 and rejuvenator). The HS1 rejuvenator is used with different contents of 0%, 4%, 12% and 20% by weight of RAP bitumen. The in-laboratory tests used to simulate the aging conditions including short-term aging, long-term aging and unaged. The rheological properties of bitumen were determined through a Dynamic Shear Rheometer (DSR) test according to AASHTO T315. The results show that, when increasing the rejuvenator content, the dynamic shear modulus G* decreases and the phase angle increases at the different aging conditions. HS1 rejuvenator is effective in reducing the hardness of bitumen blend and still has the ability to soften bitumen under long-term aging condition.