Purple tea is rich in anthocyanins, which is a valuable raw material for the production of teas with unique color or aroma, and for the production of teas with medicinal effects. In this study, nodal segments collected f-rom the imported purple tea tree were used as materials to establish a suitable protocol for micro propagation. The research results show that choosing the appropriate length of the nodal segments which is used as material is important. TDZ is not suitable for micro propagation of shoots due to the stimulation of callus formation, but BAP is effective. Nutrient-poor medium has a positive effect on root formation of in vitro purple tea shoots. At the nursery stage, type of mixture effected significally on the percentage of plant survival of tea. The results in this study can be applied for the purpose of in vitro multiplication and conservation of purple tea plants as well as Camellia species.