Nghiên cứu sử dụng Sodium Hypochilorite để thu hồi Poly(3-Hydroxybutyrate) từ chủng vi khuẩn Yangia sp. NĐ199

Chỉ số đề mục

62.13

Lĩnh vực nghiên cứu

Công nghệ sinh học công nghiệp

Dạng tài liệu

Tác giả

Đoàn Văn Thược⁽¹⁾, Lưu Thị Hồi, Trần Hữu Phong⁽²⁾

Nhan đề

Nghiên cứu sử dụng Sodium Hypochilorite để thu hồi Poly(3-Hydroxybutyrate) từ chủng vi khuẩn Yangia sp. NĐ199

Nhan đề tiếng anh

Recovery of poly(3-hydroxybutyrate) from Yangia sp. ND199 by simple digestion with sodium hypochlorite

Nguồn trích

Khoa học và Công nghệ

Năm xuất bản

2015

Số

6

Trang

706-714

ISSN

0866-708X

Từ khóa

Sodium Hypochilorite, Poly(3-Hydroxybutyrate), Yangia, Tách chiết

Từ khóa tiếng anh

Tóm tắt

Tóm tắt tiếng anh

Polyhydroxyalkanoate (PHA) is a biodegradable polymer synthesized intracellular by many microorganisms. After extraction from the cells, they possess the common features of non-toxic, biocompatible, biodegradable and recyclable. These features render them highly competitive with polypropylene or other petroleum-derived plastics, especially in medicine. The recovery of PHA from bacterial cells is the key step of PHA production process. Many methods have been used for PHA recovery; these include solvent extraction, enzymatic hydrolysis, and chemical digestion. In this study, the chemical digestion method was used for recovery of poly(3-hydroxybutyrate) (PHB) from Yangia sp. ND199. Among various chemical tested (NaOH, HCl, KOH, NaOCl and SDS), NaOCl was found as an efficient chemical for PHB recovery PHB from Yangia sp. ND199. The optimal recovery condition was a ratio of 1:1 (v/v, solution containing 100 g/l bacterial cells and solution containing 6 percent NaOCl), at 30oC for 1h. Under such conditions, a purity of 99 percent and a recovery yield of 94 percent were obtained. This purification method is simple and can be developed and used for pilot scale.

Kí hiệu kho

TTKHCNQG, CVv 5

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