Curcumin, which is traditionally known to have effects on various types of diseases in humans, is found in Curcuma longa L Previous reports indicated that the curcumin content varies between the different lines of this species, depending on other factors such as genotypes. Total curcumin content of 24 genotypes was determined using spectrometer. Group I was included 7 genotypes with low total curcumin content (0.25 - 0.62 percent) such as N4, N6, N8, N9, Nl0, N12 and N16; group II was included 17 remaining genotypes with high total curcumin content (1.80 -7.19 percent) such as Nl,N2, N3, N5, N7, Nll, N13, N14, N15, N17, N18, N19, N20, N21, N22, N23 and N24. In order to develop DNA molecular markers related to various curcumin content levels of candidate genotypes of Curcuma longa L. originated from 14 difference provinces in Vietnam, ETS (External Transcribed Spacer) region in nuclear DNA (nrDNA) and rps16 gene in chloroplasts (cpDNA) were amplified and analyzed. PCR-RFLP method was used to analyze ETS region. The results showed that low curcumin content containing genotypes gave 4 bands (0.4 kb, 0.3 kb, 0.2 kb, 0.1 kb) and high curcumin content containing genotypes gave 2 bands (0.4 kb, 0.1 kb). Analyzing of sequences of rps16 gene investigated specific markers in low curcumin containing genotypes such as AAATITITCATICGTACTCATMCTCAGTfGGGTA and in high curcumin having genotypes such as MAAATITITCATICGTACTCATMCTCMGTfGGGTM. These markers could be used for identification of turmeric genotypes containing low and high curcumin content levels, respectively.
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