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  • Công bố khoa học và công nghệ Việt Nam

34.33.15

Động vật học

Huỳnh Hoàng Như Khánh, Cao Thị Thúy Hằng(1), Phạm Đức Thịnh, Bùi Minh Lý, Lê Quang Huấn, Ngô Thị Duy Ngọc, Phan Thị Hoài Trinh, Võ Thị Diệu Trang, Lê Thị Hoa

Sự phân bố và điều kiển xúc tác của Beta-1.3-Glucanase ở động vật không xương sống biển Việt Nam

Distribution and catalytic conditions of Beta-1,3-glucanase in Vietnam marine invertebrates

Khoa học và Công nghệ (Viện Hàn lâm Khoa học và Công nghệ Việt Nam)

2015

2

139-146

0866-708X

The distribution of Beta-1.3-Glucanase in 41 species of marine invertebrates in Vietnam including echinoiderms, bivalves and gastropods was studied. After collection, species names of the invertebrates were identified and samples were kept at 4°C during transferring to the laboratory. The hepatopancreas, crystalline stalks, mesentery or eggs were separated, homogenenised in succinic buffer and concentrated to obtain a crude extract. The crude extracts were applied on a Sephadex G-25 column (1.2 x 20 cm) to remove some small molecules, then enzymological activity was screened with laminaran. The results showed that Beta-1.3-Glucanase were widespread in the animals analyzed. Catalysis conditions of enzyme were also studied in 5 species which had high activity with laminaran, it were Conus quercinus, Haliotis ovina, Perna viridis, Tapes literata and Turbo chrysotomus. These enzymes had different pH optimum and temperature optimum, but almost had high activity in pH range of 4 - 7 and at temperatures of 30 - 40°C. The time for hydrolysis was also various in range from 10 to 30 minutes.

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