Thiết kế vector trung gian Baculovirus mang hộp gen hemagglutinin (HA) để tạo Virus-like Particle của virus cúm A/H5N1 sản xuất vaccine

Chỉ số đề mục

76.03

Lĩnh vực nghiên cứu

Công nghệ sinh học trong y học

Dạng tài liệu

Tác giả

Lê Phương Hằng, Nguyễn Thị Phương, Đinh Duy Kháng, Đồng Văn Quyền⁽¹⁾

Nhan đề

Thiết kế vector trung gian Baculovirus mang hộp gen hemagglutinin (HA) để tạo Virus-like Particle của virus cúm A/H5N1 sản xuất vaccine

Nhan đề tiếng anh

Construction of baculovirus transfer vector carrying the hemaglutimin (HA) gene cassette of an A/H5N1 virus for generation of infulenza virus-like particle (VLP) vaccine

Nguồn trích

Tạp chí Công nghệ Sinh học

Năm xuất bản

2012

Số

1

Trang

59-64

ISSN

1811-4989

Từ khóa

Baculovirus, Gen hemagglutinin, Virus-like Particle, Virus cúm A/H5N1

Từ khóa tiếng anh

Tóm tắt

Tóm tắt tiếng anh

Virus-like particle (VLP)-based vaccines represent a promising approach for production of new generation vaccine with improved safety and efficacy. In order to construct a VLP-based NH5NI vaccine; herein we constructed a baculovirus transfer vector, which is able to support the independent expression of different open reading frames (ORFs), carrying a gene cassette containing hemaglutinin sequence trom the strain A/chicken/hatay/2004(H5NI). The gene encoding hemagglutinin was amplified by PCR using a specific primer set PFSP-F and HApBac-R which has initiation codon, Kozak sequence, and BamHI site at 5' of forward primer and HindIII site at 5' of reverse primer. The PCR product (HA gene cassette) was cloned into pCR2.l vector and sequenced. The HA gene cassette was then excised trom recombinant pCR2.1 vector by BamHI and HindIII and inserted into the baculovirus transfer vector (pBluBac4.5/V5-His-TOPO) previously digested by the same restriction enzymes to yield recombinant pBluBacHA plasmid. The structure and nucleotide sequences of HA gene cassette in pBluBacHA plasmid was confirmed by restriction enzyme analysis and DNA sequencing. The correct clones, in the next step, will be co-transfected with DNA of baculovirus into Spodopterafrugiperda (Sf9) cells to generate H5Nl VLP - based vaccine.

Kí hiệu kho

TTKHCNQG, CVv262

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