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  • Công bố khoa học và công nghệ Việt Nam

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Võ Hoài Bắc, Nguyễn Thị Thanh Hương, Lê Văn Trường(1)

Nghiên cứu chiết xuất polysaccharide từ lá cây xuân hoa đỏ lá đỏ Pseuderanthemum caruthersii (Seem.) Guill. var. atropurpureum (Bull.) Fosb

Optimized extraction of polysaccaride from Pseuderanthemum caruthersii (Seem.) Guill.

Sinh học

2018

1

162-167

0866-7160

Tách chiết, xác định hàm lượng và tinh sạch sơ bộ polysaccharide từ lá cây P. carruthersii var. atropurpureum. Hàm lượng polysaccharide trong lá cây đạt 11,63% ± 0,38 trọng lượng khô. Các điều kiện chiết rút polysaccharide thích hợp đã được xác định nước là dung môi thích hợp, nhiệt độ chiết rút 60°C, tỷ lệ nguyên liệu/dung môi (1g mẫu khô/25ml), thời gian chiết rút 15 giờ. Chế phẩm polysaccharide đã được tinh sạch bằng TCA 10%, có độ sạch đạt 71,47% ± 0,94). Chế phẩm polysaccharide bán tinh sạch thu được 2,82 ± 0,24 g/100 g nguyên liệu khô.

Pseuderanthemum carruthersii (Seem.) Guill. var. atropurpureum (Bull.) Fosb is a tree native of Vietnam, this medicinal leaf has a high viscosity that has been used to heal wounds, heal the sores, stop bleeding... Polysaccharide has attracted great attentions for its benefits to human health. Polysaccharide from natural sources have diverse anti-inflammatory, anticoagulant and wound healing activities. Polysaccharide is not only valuable in medicine, also widely used in foodstuffs such as gel thickening or emulsifying agents, emulsifiers, fillers. The polysaccharide content in P. carruthersii var atropurpureum leaves was 11.63% ± 0.375 in dry weight. The appropriate polysaccharide extraction conditions were determined water, material/solvent ratio (1g/25ml), extracted temperature of 60°C, extraction time 15 hours. The polysaccharide composition was purified by TCA 10%, with a purity of 71.47% ± 0.94.

TTKHCNQG, CVv 27

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