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  • Công bố khoa học và công nghệ Việt Nam

76.03

Công nghệ sinh học trong y học

Nguyễn Quang Đông, Tran Viet Hung(1)

Nghiên cứu ảnh hưởng của thời gian bảo quản tới chất lượng khối tiểu cầu

Studying on effectiveness of duration of conservation to quality of blood plates

Y học thực hành

2014

9

17-20

1859-1663

Purpose: To studying some changes of biochemistry, morphology and function of plates in conservative time. Subjects and methods: 30 plates units were splitted by apheresis named haemonetic machine, keep in 22 C degree and shaking. Collection of data: number of plates, MVP, DPV, PLCR, pH, glucose, lactate and platelet aggregation at day 1, day 3, day 5 of duration of conservation. Results: Number of plates at the day 1 is 1401 + or - 331, day 2 is 1365 + or - 311 and day 3 is 1196 + or - 234 G/I. Platelet index MPV, DPV, P-LCR increased at the :fd conservative day. pH of blood plates at day 1,3,5 correspondence is 6.99 + or - 0.11; 6.88 + or - 0.16 or 6.83 + or - 0.18. Concentration of glucose at day 1,3,5 is 19.25 + or - 2.97, 10.80 + or - 3.92 and 7.14 + or - 5.17 mmol/l. Level of lactate is 3.21 + or - 0.87, 14.07 + or - 4.39 and 18.63 + or - 6.05 mmol/l at day 1,3,5. A close correlation between pH and platelet index: MPV, DPV, P-LCR. Platelet aggregation with collagen is 73.56 + or - 21.91; 49.77 + or - 27.71; 10.17 + or - 11.90 percent; with ADP is 27.20 + or - 9.07; 8.17 + or - 6.62; 6.93 + or - 3.78 percent at day 1,3,5. Conclusion: There are reduce of platelet number, changes of platelet morphology (increase of MPV, DPV, P-LCR) and close correlation with decrease of pH of blood plates in duration of conservation. Platelet aggregation with DAP and collagen is also decreased following conservative time.

TTKHCNQG, CVv 57