With a view to becoming the national shrimp capital, authorities in Bac Lieu has oriented the development and applications of science and technology in shrimp industry. Bac Lieu province is known as a one of the most developed aquaculture areas in the Mekong Delta region with various systems such as semi-intensive, intensive, and super-intensive shrimp farming. Therefore, the aim of this study was to assess the quality of water supply resources surrounding shrimp culture ponds to provide fundamental understanding about the spatial and seasonal variations of water quality in Bac Lieu where the shrimp culture has been intensified. Water samples were monthly collected at 5 sites in key features of estuaries in the shrimp culture area of the province over a year. Results showed that the water quality in the shrimp farming area is fewer fluctuations, still qualified the requirements of the national standards, and suitable as water supply for shrimp culture in the research area. However, it could be noted that concentrations of TSS, H2S, and PO4 3- were relatively high compared to the standards for marine shrimp. Considering the BL1 (Nha Mat estuary - Bac Lieu city) and BL5 (Ganh Hao estuary - Dong Hai) sites, most water quality parameters were highly fluctuated among the seasons. Additionally, poor water quality occurred in the dry and dry-rainy seasons at the Ganh Hao estuary (site BL5).