Sự phát sinh cơ quan và nhân giống vô tính cây kiwi (Actinidia deliciosa) thông qua nuôi cấy mô lá in vitro

Chỉ số đề mục

62.33

Lĩnh vực nghiên cứu

Công nghệ sinh học công nghiệp

Dạng tài liệu

Tác giả

Dương Tấn Nhựt⁽¹⁾, Hoàng Văn Cương, Lê Thị Tuyết, Nguyễn Bá Nam, Nguyễn Thị Hướng, Lê Kim Cương, Hoàng Xuân Chiến, Nguyễn Phúc Huy, Nguyễn Hữu Lễ, Thái Xuân Du

Nhan đề

Sự phát sinh cơ quan và nhân giống vô tính cây kiwi (Actinidia deliciosa) thông qua nuôi cấy mô lá in vitro

Nhan đề tiếng anh

Organogenesis and micropropagation of Kiwi (Actinidia deliciosa) via in vitro leaf cultrure

Nguồn trích

Công nghệ sinh học

Năm xuất bản

2013

Số

3

Trang

495-503

ISSN

1811-4989

Từ khóa

Cây Kiwi, Actinidia deliciosa, Nhân nhanh chồi, Phát sinh cơ quan, Nhân giống vô tính, Nuôi cấy in vitro, Mô lá

Từ khóa tiếng anh

Tóm tắt

Tóm tắt tiếng anh

Kiwi (Actinidia deliciosa) is known as a kind of medicinal plant, as well as fruit with high content of vitamins and minerals. Currently, Kiwi has been grown and produced in many countries around the world; however, it has not been grown popularly in Vietnam. In order to create the initial source for the propagation, in this study, the authors have successfully regenerated Kiwi via organogenesis from in vitro leaf culture. After 6 weeks, the authors found that leaf samples were cultured on MS medium supplemented with 30 g/l sucrose, 8 g/l agar, 0.02 mg/l NAA and 0.5 mg/l TDZ offered the highest number of shoots (13.33 shoots/sample), callus induction from leaf sample can be observed only on MS medium supplemented with kinetin and shoot formation on MS medium with high concentration of BA. The highest shoot formation number was achieved when they were subcultured on MS medium culture supplemented with 30 g/l sucrose, 8 g/l agar and 0.01 mg/l TDZ (8.33 shoots/sample after 6 weeks of culture). The shoots then were rooted on MS medium supplemented with 30 g/l sucrose, 8 g/l agar and 0.05 mg/l NAA. The Kiwi plantlets were able to survive well when grown in the system covered with nylon (98.5 percent), to grow and to develop well in the nursery.

Kí hiệu kho

TTKHCNQG, CVv 262

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