Elevated levels of HVA and VMA in urine are often indicated as the presence of neural crest derived tumors such as neuroblastoma in children and ganglioneuroma in adults. A simple method of measuring urine HV A and VMA levels simultaneously would reduce the time and cost of diagnosis and treatment of these neural crest derived tumors. The aim of this study was to develop and validate a method to measure the concentrations of HV A and VMA simultaneously by GCMS. the authors have successfully developed a rapid and simple method for simultaneously quantizing the concentrations of HVA and VMA in urine using GCMS. Urine samples were collected and HVA and VMA were extracted and derived into their respective di- and tri(trimethylsilyl) derivatives. The derivatives were separated and quantified by SIM (Selected Ion Monitoring) on GCMS system. The measuring limits for measuring both HVA and VMA in urine by GCMS were 0.9 umol/L. The linearity range of HVA was 5.68 to 192.9 umol/L and of VMA was 3.2 to 221 umol/L. The calculated total errors were smaller than the acceptable total values. Therefore, this method allows clinicians to measurea accurately the concentrations of urinary VMA and HVA simultaneously using GCMS. This simple technique can be applied to the routine diagnosis and treatment monitoring of neuroblastoma.
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