Ecotourism is a form of tourism based on nature and indigenous culture that involves education and interpretation of the natural environment and improving the well-being of the local people. With the advantages of a large campus, spacious and modern facilities, especially an experimental forest with an area of over 130 hectares with high biodiversity value, Vietnam National University of Forestry is considered as a suitable place for environmental interpretation and ecotourism development. Secondary data collection, interview, and field survey methods have been applied in this research. As a results, an ecotourism route at Vietnam National University of Forestry with a total length of 5.26 km and seven points of environmental interpretation has been established. Interview results show that the ecotourism route is really need to help students and visitors discover the value of biodiversity resources in the experimental mountain forest and environmental interpretation activities. Simultaneously, this research proposed some solutions to support the operation and management of ecotourism routes, including human resource, technical, and promotion solutions.