During survey on entomopathogenic nematodes carried out in 2004, a new record of the genus Steinernema (S-XT) was isolated from a soil sample that collected from Xuan Trach, Quang Binh province. The morphological and molecular identification of S-XT isolate have revealed scientific name as Steinernema guangdongense Qiu, Fang the presence of a small double flapped epiptygma, a small projection on the dorsal side of the tail tips and its prominent post-anal swelling part, which is typical for S. guangdongense. In males, the spicules paired, brown color, head (manubrium) of spicules with rounded anterior end, almost continuous with shaft, except body length of females at the first generation always larger. The morphology, morphometries and molecular characterization based on Vietnamese specimens were supplemented data for the redescription of S. guangdongense and confirmed it belonging to "glaseri" group with the body length of infective juveniles fluctuated between 1,055 11m and 1,063 11m and eight lines in lateral fields. The newly recorded species is also close to S. longicaudum, which was also recorded from Vietnam but differed from it by having another morphological structure of the spicules apparatus, present of the paired epiptigma in the female and the numbers of the lateral fields in the infective juveniles.
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